Single molecule microscopy / spectroscopy

  Picture shows optic table Fitter Typical view on the optical excitation light path of a fluorescence microscope.

In our lab we operate two fluorescence microscopes for single molecule detection. In addition to a dual color confocal microscope (MicroTime 200, PicoQuant) with pulsed excitation at 485 and 638 nm and two detection channels, we are currently setting up a widefield/TIRF microscope with dual color (488 nm, 640 nm) excitation. By employing single-photon counting with sub nanosecond time resolution we make use of extensive multi-parameter fluorescence analysis. Major methods applied in our group are single molecule Förster resonance energy transfer (smFRET), fluorescence correlation spectroscopy (FCS), time resolved anisotropy decay (TRA) and photo-induced electron transfer (PET). In this respect many of our activities are related to methodical developments, both on the experimental side and by improving data analysis tools.

 

Publications

J. Fitter, A. Katranidis, T. Rosenkranz, D. Atta, R. Schlesinger, G. Büldt
Single molecule fluorescence spectroscopy: A tool for protein studies approaching cellular environmental conditions .
Soft Matter, 7, 1254-1259, (2011)

D. Kempe, A. Schöne, J. Fitter, and M. Gabba
Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy
J. Phys. Chem. B, 119, 4668-4672, (2015)